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Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material
Published on: August 1, 2018
Hormone receptor expression in breast cancer: postanalytical issues
Ming Lee1, Cheok Soon Lee, Puay Hoon Tan
1Department of Pathology, Singapore General Hospital, Singapore.
Journal of Clinical Pathology
|March 26, 2013
Summary
Accurate interpretation of oestrogen receptor (ER) and progesterone receptor (PR) status in breast cancer pathology is vital for treatment. Postanalytical factors, including tumor characteristics, can affect ER/PR testing accuracy.
Area of Science:
- Oncology
- Pathology
- Molecular Biology
Background:
- Hormone receptor (HR) status, including oestrogen receptor (ER) and progesterone receptor (PR), is crucial for breast cancer diagnosis, treatment selection, and prognosis.
- Accurate laboratory testing for ER/PR requires meticulous preanalytical, analytical, and postanalytical procedures.
Purpose of the Study:
- This report focuses on postanalytical challenges in the pathological interpretation of ER and PR status in breast cancer.
- To highlight factors beyond the direct pathological assessment that influence the accuracy and reliability of hormone receptor expression testing.
Main Methods:
- Review of postanalytical issues in breast cancer pathology reporting.
- Discussion of factors affecting the sensitivity, specificity, and dynamic range of ER/PR assays.
Main Results:
- Pathologist interpretation of ER and PR status can be influenced by various factors.
- Tumor characteristics, heterogeneity, tumor progression, and interacting molecules can impact hormone receptor expression levels and responsiveness.
Conclusions:
- Ensuring accuracy in ER/PR testing requires addressing postanalytical interpretation challenges.
- Continuous engagement in quality assurance, slide exchanges, and staying updated on clinical trial data is essential for managing hormone receptor-positive breast cancer.
