Related Experiment Video
Updated: May 13, 2026
Spontaneous Formation and Rearrangement of Artificial Lipid Nanotube Networks as a Bottom-Up Model for Endoplasmic Reticulum
Published on: January 22, 2019
Single step bottom-up process to generate solid phospholipid nano-particles
Ulla Brinkmann-Trettenes1, Sabine Barnert, Annette Bauer-Brandl
1Institute of Physics, Chemistry and Pharmacy, University of Southern Denmark , Odense M , Denmark and.
Context:
The particularity of the Nano Spray Dryer B-90 is the nozzle containing a mesh vibrating at ultrasonic frequency.
Objective:
To study process parameters and processability of crude phospholipid dispersions, in particular the effect of concentration and mesh aperture on both particle size of the dry solid phospholipid nano-particles and on the re-dispersed powder.
Materials And Methods:
Phospholipid dispersions containing trehalose as a stabilizer were spray dried. Particle size distributions of dry powders were evaluated by SEM micrographs and by PCS and cryo-TEM for the re-dispersed particles.
Results:
Spray drying of crude liposome dispersions revealed solid phospholipid nano-particles. Aperture of nozzle mesh and concentration of the dispersions, respectively, both increased the size of solid phospholipid nano-particles. For crude dispersions, an upper limit with respect to processability was found close to below 10% (m/m) even if the crude dispersion was passed along the mesh several times; however, more effective dispersing methods such as pre-sonication can push the limit of processability to higher values.
Discussion And Conclusion:
The nano spray dryer is capable of spray drying crude dispersions of phospholipids in concentrations below 10% (m/m) generating solid phospholipid nano-particles relevant for pulmonary delivery. Re-dispersion of spray dried powder reveals liposomes.
