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Updated: May 12, 2026

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
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Improvement of pronuclear injection-based targeted transgenesis (PITT) by iCre mRNA-mediated site-specific

Masato Ohtsuka1, Hiromi Miura, Hideki Hayashi

  • 1Division of Basic Medical Science and Molecular Medicine, Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan. masato@is.icc.u-tokai.ac.jp

Transgenic Research
|March 27, 2013
PubMed
Abstract

No abstract available in PubMed .

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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