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Related Experiment Video

Updated: May 12, 2026

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
09:32

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

Published on: October 23, 2016

[A method for isolating and identifying newborn rat sino-atrial node cells for patch-clamp study].

Yanli Wang1, Ruxiu Liu, Yu Liu

  • 1Department of Cardiology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, China. wangyanlihappy@yahoo.com.cn

Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University
|March 27, 2013
PubMed
Summary
This summary is machine-generated.

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Enzyme digestion with differential attachment effectively isolates sino-atrial node cells from newborn rats. This method enhances cell viability and patch clamp success rates compared to the conventional 5-bromodeoxyuridine technique.

Area of Science:

  • Cardiovascular Physiology
  • Cell Biology
  • Electrophysiology

Background:

  • Sino-atrial node (SAN) cells are crucial for heart rhythm regulation.
  • Accurate isolation of SAN cells is vital for electrophysiological studies.
  • Conventional methods for SAN cell isolation can be complex and impact cell viability.

Purpose of the Study:

  • To compare two methods for isolating SAN cells from neonatal rats: enzyme digestion with differential attachment versus the conventional 5-bromodeoxyuridine (5-BrdU) method.
  • To evaluate the efficacy of each method for patch clamp studies.

Main Methods:

  • Neonatal Wistar rat SAN tissue was processed using two isolation techniques.
  • Isolated cells were analyzed for morphology, structure, beat frequency, and patch clamp suitability.

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Last Updated: May 12, 2026

Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice
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Methods for the Isolation, Culture, and Functional Characterization of Sinoatrial Node Myocytes from Adult Mice

Published on: October 23, 2016

Microelectrode Array Recording of Sinoatrial Node Firing Rate to Identify Intrinsic Cardiac Pacemaking Defects in Mice
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  • Atrial cells isolated via enzyme digestion with differential attachment served as controls.

    • Main Results:

    • Both methods yielded spindle-shaped SAN cells capable of spontaneous action potentials.
    • The enzyme digestion with differential attachment method resulted in a higher proportion of spindle-shaped cells.
    • Cells isolated with enzyme digestion and differential attachment demonstrated superior viability and a higher success rate in patch clamp experiments.

    Conclusions:

    • Enzyme digestion with differential attachment is a viable method for isolating SAN cells.
    • This technique offers a simpler procedure with preserved cell viability.
    • The method is advantageous for subsequent patch clamp electrophysiological studies.