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Monitoring M-BCR-ABL expression level in CML patients by RQ-PCR: experience of a single Center
Annamária Szántó1, Zsuzsanna Pap, I Benedek
1University of Medicine and Pharmacy of Targu Mures, Romania.
Unlabelled:
Chronic myelogenous leukemia (CML) is characterized by the Philadelphia chromosome and the BCR-ABL fusion gene that encodes an abnormal tyrosine kinase. Development of specific tyrosine kinase inhibitors completely changed the management of these patients.
Materials And Methods:
Between April 2008 and July 2012, at the Molecular Biology Laboratory, University of Medicine and Pharmacy of Targu Mures, Romania, we monitored the M-BCR-ABL transcript level by real time quantitative PCR in case of 15 CML patients diagnosed at the Hematology and Transplant Center of Targu Mures.
Results:
Modification of M-BCR-ABL expression level shows statistically significant correlation (p=0.013) with the clinical course of these patients.
Conclusions:
Molecular biology techniques have an important role in monitoring CML patients and regular analysis is recommended.
