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Related Concept Videos

Patch Clamp01:18

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: May 12, 2026

One-channel Cell-attached Patch-clamp Recording
13:07

One-channel Cell-attached Patch-clamp Recording

Published on: June 9, 2014

Recording single-channel currents using "smart patch-clamp" technique.

Anamika Bhargava1, Julia Gorelik

  • 1Department of Cardiovascular Sciences, National Heart and Lung Institute, Imperial College London, London, UK.

Methods in Molecular Biology (Clifton, N.J.)
|March 27, 2013
PubMed
Summary
This summary is machine-generated.

A new "Smart patch-clamp" technique allows researchers to study ion channels in cell surface microdomains. This method combines patch-clamp with Scanning Ion Conductance Microscopy (SICM) for precise measurements.

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Last Updated: May 12, 2026

One-channel Cell-attached Patch-clamp Recording
13:07

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Published on: June 9, 2014

Whole-cell Patch-clamp Recordings in Brain Slices
07:23

Whole-cell Patch-clamp Recordings in Brain Slices

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A Computer-assisted Multi-electrode Patch-clamp System
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A Computer-assisted Multi-electrode Patch-clamp System

Published on: October 18, 2013

Area of Science:

  • Cell Biology
  • Biophysics
  • Cardiology

Background:

  • Cell surface microdomains are crucial for cellular signaling.
  • Ion channel localization within these microdomains dictates signaling pathways.
  • Excitable cells, like cardiomyocytes, rely on spatially coupled ion channels for function.

Purpose of the Study:

  • To introduce and validate a novel advanced patch-clamp technique called "Smart patch-clamp."
  • To enable the study of functional ion channels within cell surface microdomains.
  • To focus on recording L-type calcium channel currents in adult rat cardiomyocyte T-tubules.

Main Methods:

  • The "Smart patch-clamp" technique integrates conventional patch-clamp with Scanning Ion Conductance Microscopy (SICM).
  • SICM utilizes a glass micropipette to generate high-resolution cell surface topography images.
  • The same micropipette serves as a patch-clamp pipette for targeted ion channel recordings.

Main Results:

  • The Smart patch-clamp technique successfully identified and recorded single channel L-type calcium channel currents.
  • Recordings were specifically obtained from the T-tubules of adult rat cardiomyocytes.
  • The method allows for precise localization of ion channel activity within specific cell surface microdomains.

Conclusions:

  • Smart patch-clamp is a powerful new tool for investigating ion channel function in cellular microdomains.
  • This technique enhances our understanding of ion channel localization and its impact on cellular signaling.
  • The study demonstrates the applicability of Smart patch-clamp in excitable cells like cardiomyocytes.