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Capillary Electrophoresis: Applications

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...

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On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids

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Published on: March 2, 2012

Microfluidic isotachophoresis: a review.

Petr Smejkal¹, Danny Bottenus, Michael C Breadmore

¹ACROSS and School of Chemistry, University of Tasmania, Hobart, Australia.

Electrophoresis

|March 27, 2013

Summary

Microfluidic isotachophoresis (μ-ITP) offers advantages like analyte preconcentration and matrix removal in miniaturized devices. This review covers historic and recent developments in experimental and computational μ-ITP for diverse applications.

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Area of Science:

Analytical Chemistry
Separation Science
Microfluidics

Background:

Electromigration techniques, including capillary electrophoresis (CE) and isotachophoresis (ITP), are suitable for miniaturization in microfluidic devices.
Microfluidic ITP (μ-ITP) has resurged due to its steady-state boundary, self-focusing, and analyte preconcentration capabilities, alongside matrix component removal.

Purpose of the Study:

To provide a comprehensive overview of microfluidic isotachophoresis (μ-ITP) developments.
To highlight recent advancements in both experimental and computational ITP.
To discuss future trends in chip-based ITP.

Main Methods:

Review of historic and recent developments in experimental and computational ITP.
Discussion of various μ-ITP formats including PC simulations, analytical, preconcentration, transient, peak mode, gradient elution, and free-flow ITP.
Compilation of experimental conditions from over 50 publications for real-world sample applications.

Main Results:

Detailed examination of diverse μ-ITP techniques and their applications.
Inclusion of computational modeling and simulation aspects.
Synthesis of experimental data for practical μ-ITP implementations.

Conclusions:

μ-ITP is a powerful technique for miniaturized analytical systems.
Recent advancements have expanded the scope and capabilities of μ-ITP.
Future trends point towards further integration and application of μ-ITP in complex sample analysis.