Related Experiment Video
Updated: May 12, 2026
3D Human Myocardial Tissue Generation Using Melt Electrospinning Writing of Polycaprolactone Scaffolds and hiPSC-Derived Cardiac Cells
Published on: March 28, 2025
Poly(ε-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal
Spencer W Crowder1, Yi Liang, Rutwik Rath
1Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.
Aim:
To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs).
Materials & Methods:
Electrospun scaffolds of poly(ε-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs.
Results:
Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(ε-caprolactone) scaffolds without carbon nanotube and azacytidine.
Conclusion:
This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium.
