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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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Related Experiment Video

Updated: May 12, 2026

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Generation, Maintenance, and Characterization of Human Pluripotent Stem Cell-derived Intestinal and Colonic Organoids

Published on: July 9, 2021

A gutsy task: generating intestinal tissue from human pluripotent stem cells.

Stacy R Finkbeiner¹, Jason R Spence

¹Division of Gastroenterology, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA.

Digestive Diseases and Sciences

|March 28, 2013

Summary

Researchers review advances in generating human intestinal tissue from pluripotent stem cells. This method offers new ways to study intestine development and compare it with primary intestinal cultures.

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Area of Science:

Gastroenterology and Developmental Biology
Stem Cell Biology and Regenerative Medicine

Background:

Recent years have seen significant progress in understanding intestine development and stem cell dynamics.
Novel techniques enable culturing of primary intestinal epithelium and de novo generation of human intestinal tissue.

Purpose of the Study:

This review focuses on the directed differentiation of human pluripotent stem cells into intestinal tissue.
It highlights novel applications of this engineered tissue.
The study compares this system with primary intestinal epithelial cultures.

Main Methods:

Review of recent literature on directed differentiation of human pluripotent stem cells into intestinal tissue.
Analysis of novel applications and comparative studies of engineered intestinal tissue versus primary cultures.

Main Results:

Directed differentiation of human pluripotent stem cells provides a viable model for intestinal tissue.
Engineered intestinal tissue offers new avenues for research in development, disease modeling, and regenerative medicine.
Comparison with primary cultures reveals distinct advantages and limitations of each system.

Conclusions:

Human pluripotent stem cell-derived intestinal tissue represents a significant advancement in the field.
This model system holds great promise for future research in intestinal biology and therapeutics.
Further research is warranted to optimize differentiation protocols and explore clinical applications.