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Nordihydroguaiaretic acid attenuates the oxidative stress-induced decrease of CD33 expression in human monocytes.

Silvia Guzmán-Beltrán1, José Pedraza-Chaverri, Susana Gonzalez-Reyes

  • 1Departamento de Microbiología en Investigación, Instituto Nacional de Enfermedades Respiratorias, Ismael Cosío Villegas, Calzada de Tlalpan 4502, Sección XVI, 14080 México, DF, Mexico.

Oxidative Medicine and Cellular Longevity
|March 28, 2013
PubMed
Summary
This summary is machine-generated.

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Nordihydroguaiaretic acid (NDGA) protects human monocytes from oxidative stress. NDGA preserves CD33 expression and reduces cell death caused by reactive oxygen species, highlighting its antioxidant benefits.

Area of Science:

  • Biochemistry
  • Immunology
  • Pharmacology

Background:

  • Nordihydroguaiaretic acid (NDGA) is a natural lignan from Larrea tridentata with known antioxidant properties.
  • CD33 is a cell surface receptor expressed on human monocytes (MNs).
  • Oxidative stress can negatively impact cellular functions and receptor expression.

Purpose of the Study:

  • To investigate the effect of NDGA on oxidative stress-induced downregulation of CD33 in human monocytes.
  • To assess NDGA's protective role against oxidative damage and cell death.

Main Methods:

  • Oxidative stress was induced in human monocytes using iodoacetate (IAA) or hydrogen peroxide (H(2)O(2)).
  • Reactive oxygen species (ROS) production and cell viability were measured.
  • The expression of CD33 was evaluated under oxidative stress conditions with and without NDGA treatment.

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  • Experiments were also conducted on the THP-1 cell line.

    • Main Results:

    • NDGA significantly attenuated toxicity and reactive oxygen species (ROS) production induced by IAA or H(2)O(2).
    • NDGA prevented the decrease in CD33 expression caused by oxidative stress in human monocytes.
    • NDGA (20 μM) protected THP-1 cells from cell death induced by IAA or H(2)O(2).

    Conclusions:

    • NDGA exhibits a protective effect on CD33 expression in human monocytes under oxidative stress.
    • The protective effect of NDGA is linked to its potent antioxidant activity.
    • NDGA demonstrates potential therapeutic value in mitigating oxidative stress-related cellular damage.