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Related Experiment Video

Updated: May 12, 2026

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency

Published on: June 16, 2011

Beta-actin variant is necessary for Enterovirus 71 replication.

Yan Long Edmund Lui¹, Zhiyang Lin, Jia Jun Lee

¹Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore.

Biochemical and Biophysical Research Communications

|March 29, 2013

Summary

This summary is machine-generated.

Enterovirus 71 (EV71), a cause of Hand, Foot, and Mouth Disease (HFMD), utilizes beta-actin and annexin 2 for cellular transport. This finding aids in understanding EV71 pathogenesis and disease establishment.

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Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

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Area of Science:

Virology
Cell Biology
Proteomics

Background:

Enterovirus 71 (EV71) is a primary cause of Hand, Foot, and Mouth Disease (HFMD).
EV71 infection can lead to severe neurological complications, including encephalitis and paralysis.
EV71 is endemic in the Asia-Pacific region, causing significant public health concerns.

Purpose of the Study:

To investigate the proteomic differences between EV71-susceptible and EV71-resistant human Rhabdomyosarcoma (RD) cells.
To identify host cell proteins involved in EV71 infection and pathogenesis.

Main Methods:

Proteomic comparison of EV71-susceptible and EV71-resistant RD cell lines.
Analysis of protein expression and variants, focusing on beta-actin and associated proteins.

Main Results:

Significant differences in beta-actin variants were observed between susceptible and resistant RD cells.
Beta-actin, in conjunction with annexin 2, appears crucial for the vesicular transport of EV71.
These findings suggest a role for actin in EV71 pathogenesis and disease establishment.

Conclusions:

Beta-actin and annexin 2 are implicated in the cellular mechanisms of EV71 infection.
Understanding these host-pathogen interactions can inform strategies for combating HFMD and EV71-related neurological diseases.