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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
Published on: May 21, 2020
Tissue-specific transcriptomic profiling of Sorghum propinquum using a rice genome array.
Ting Zhang1, Xiuqin Zhao, Liyu Huang
1Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing, China.
Plos One
|March 29, 2013
Summary
This study analyzed gene expression in Sorghum propinquum rhizomes, identifying key genes and cis-elements involved in growth. Findings highlight phytohormones as crucial regulators for developing sustainable sorghum cropping systems.
Area of Science:
- Plant Genomics
- Functional Genomics
- Crop Science
Background:
- Sorghum (Sorghum bicolor) is a vital cereal crop.
- Sorghum propinquum, a wild relative, possesses rhizomes and offers potential for sustainable agriculture.
- Understanding rhizome development mechanisms is key for improving grain, forage, and bioenergy systems.
Purpose of the Study:
- To investigate tissue-specific gene expression in S. propinquum rhizomes.
- To identify molecular mechanisms underlying rhizome growth and development.
- To provide functional candidates for genes controlling rhizome development.
Main Methods:
- Utilized a whole rice genome oligonucleotide microarray for gene expression profiling.
- Analyzed tissue-specific gene expression in S. propinquum rhizome tips and internodes.
- Performed Gene Ontology (GO) analysis and comparative genomics.
Main Results:
- Identified 548 tissue-enriched genes in S. propinquum rhizomes.
- Found 31 unique genes in rhizome tips and 114 in internodes.
- Discovered prevalent cis-elements (ABA, GA-responsive) and confirmed phytohormones (ABA, GA, SA) as key regulators.
Conclusions:
- Phytohormones play a critical role in regulating gene expression during sorghum rhizome development.
- Identified potential genes for rhizome growth and development through co-localization with QTLs.
- Results contribute to developing sustainable sorghum cropping systems.
