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siRNA - Small Interfering RNAs

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Related Experiment Video

Updated: May 12, 2026

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Long-term Silencing of Intersectin-1s in Mouse Lungs by Repeated Delivery of a Specific siRNA via Cationic Liposomes. Evaluation of Knockdown Effects by Electron Microscopy

Published on: June 21, 2013

Lipoplexes versus nanoparticles: pDNA/siRNA delivery.

Bharat Khurana¹, Amit K Goyal, Abhishek Budhiraja

¹Department of Pharmaceutics, Nanomedicine Research Centre, Indo-Soviet Friendship College of Pharmacy, Moga, Punjab, India.

|March 30, 2013

Summary

Cationic liposomes and chitosan nanoparticles effectively deliver small interfering RNA (siRNA) for genetic disorder treatment. Liposomes demonstrated superior gene silencing efficiency compared to chitosan nanoparticles in mammalian cells.

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Area of Science:

Biotechnology
Nanomedicine
Molecular Biology

Background:

Small interfering RNA (siRNA) holds therapeutic potential for genetic disorders.
Systemic delivery of siRNA is hindered by degradation, poor cellular uptake, and limited stability.
Efficient delivery systems are crucial for enhancing siRNA transfection and therapeutic duration.

Purpose of the Study:

To develop and compare chitosan nanoparticles and cationic liposomes as siRNA delivery vehicles.
To evaluate the transfection efficiency and therapeutic activity of these nanocarriers in a mammalian cell line.

Main Methods:

Plasmid DNA (pEGFPN3) expressing green fluorescent protein (GFP) was used as a reporter gene.
Chitosan nanoparticles/polyplexes and cationic liposomes/lipoplexes were characterized (zeta potential, size, morphology).
DNA retardation, transfection efficiency, and cytotoxicity were assessed in HEK 293 cells.
Co-transfection with GFP-specific siRNA was performed to evaluate gene silencing.

Main Results:

Both chitosan nanoparticles and cationic liposomes served as efficient siRNA delivery vehicles.
Cationic liposomes achieved approximately 70% suppression of GFP expression.
Chitosan nanoparticles achieved approximately 57% suppression of GFP expression.
Cationic liposomes exhibited superior performance over chitosan nanoparticles in gene silencing.

Conclusions:

Cationic liposomes are more effective than chitosan nanoparticles for siRNA delivery in mammalian cells.
These findings highlight the potential of cationic liposomes as a promising carrier for siRNA-based therapeutics.