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Related Experiment Video

Updated: May 12, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

False decrease of HBsAg S/CO values in serum with high-concentration rheumatoid factors.

Lei Xu¹, Xueping Wang, Ronghong Ma

¹Laboratory Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.

Clinical Biochemistry

|March 30, 2013

Summary

This summary is machine-generated.

High-concentration rheumatoid factors (RFs) can lead to falsely decreased serum Hepatitis B surface antigen (HBsAg) signal-to-cutoff (S/CO) values when using one-step ELISA testing.

Related Experiment Videos

Last Updated: May 12, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

Area of Science:

Clinical Chemistry
Immunology
Hepatology

Background:

Hepatitis B virus (HBV) infection is a global health concern.
Accurate detection of Hepatitis B surface antigen (HBsAg) is crucial for diagnosis and management.
High levels of rheumatoid factors (RFs) can interfere with immunoassays.

Purpose of the Study:

To evaluate the impact of high-concentration RFs on HBsAg detection using one-step ELISA.
To determine if RFs cause a false decrease in HBsAg signal-to-cutoff (S/CO) values.

Main Methods:

Serum HBsAg was analyzed in 100 RF-positive samples using one-step ELISA.
Dilution experiments were performed on HBsAg-negative sera with high RFs.
Artificial serum models with HBsAg and high RFs were created.

Main Results:

One-step ELISA showed decreased HBsAg positivity rates with increasing dilution in RF-positive samples.
Artificial serum models exhibited significantly lower median S/CO values for HBsAg in the presence of high RFs compared to controls.
Two-step ELISA confirmed 100% HBsAg positivity without dilution, highlighting the interference.

Conclusions:

High-concentration RFs interfere with one-step ELISA, causing a false decrease in HBsAg S/CO values.
This interference can lead to potential underestimation of HBsAg in patients with high RF levels.
Careful consideration of RF interference is necessary when interpreting one-step ELISA results for HBsAg.