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Updated: May 12, 2026

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
Published on: May 4, 2018
Expanded genetic code for the engineering of ribosomally synthetized and post-translationally modified peptide
1Technische Universität Berlin (Berlin Institute of Technology), Department of Chemistry, Biocatalysis Group, Müller-Breslau-Straße 10, D-10623 Berlin, Germany. budisa@biocat.tu-berlin.de
Abstract:
The number of constituent amino acids in ribosomally synthetized and post-translationally modified peptide natural products (RiPPs) is restricted to the 20 canonical amino acids. Microorganisms with an engineered genetic code are capable of delivering the biological, chemical, or physical properties of many unnatural or synthetic noncanonical amino acids, ncAAs (in different combinations of their numbers and chemistry) precisely defined by the chemist at the bench. In this way, post-translational modifications (PTMs) which make RiPPs chemically extremely rich can be augmented by the co-translational insertion of ncAAs. This will dramatically expand the chemical and functional space of these molecules and enable the design of novel and unique sequence combinations with improved specificity, stability, membrane permeability and even better oral availability.
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