Related Experiment Video
Updated: May 12, 2026

Formulations for Freeze-drying of Bacteria and Their Influence on Cell Survival
Published on: August 3, 2013
Effect of buffer systems and disaccharides concentration on Podoviridae coliphage stability during freeze drying and
1Center for Research and Development in Food Cryotechnology-CIDCA-CONICET-UNLP, CCT La Plata, La Plata, Argentina. cdini@biol.unlp.edu.ar
Abstract:
The aims of this study were to determine the stability of Podoviridae coliphage CA933P during lyophilization and storage in different media, and to establish similarities between the results obtained and those expected through mechanisms described for proteins stabilization during freeze-drying. PBS and SM buffer were assayed as lyophilization media. The effect of inorganic salts concentration as well as the addition of disaccharides on phage stability during freeze-drying and storage was also studied. The addition of low sucrose concentration (0.1 mol l⁻¹) to SM buffer stabilized phage during freezing and drying steps of the lyophilization process, but higher sugar concentrations were detrimental to phage stability during freeze-drying. Sucrose stabilized phage during storage for at least 120 days. The lyoprotective effect of low concentrations of disaccharides during the drying step of the lyophilization of proteins as well as the stabilization of the freeze-dried product in time correlated with the results obtained for phage CA933P.
Related Concept Videos
Drug Product Stability
Buffer Effectiveness
The buffer capacity is the amount of acid or base that can be added to a given volume...
The Colloidal State
Protein Buffers in Blood Plasma and Cells
Certain amino acids can exist in a zwitterion state at a...

