Related Experiment Video

Updated: May 12, 2026

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein
10:25

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

Published on: November 10, 2012

Role of the ubiquitin proteasome system in the heart

Julia Pagan1, Tiffany Seto, Michele Pagano

  • 1Department of Translational Medical Sciences, Via Sergio Pansini, 5, 80131 Naples, Italy.

Circulation Research
|March 30, 2013
PubMed

Insights

Proper protein turnover is crucial for heart health. This review explores how the ubiquitin proteasome system impacts heart disease and discusses potential therapeutic targets for improving cardiac function.

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • Protein Degradation

Background:

  • Protein turnover is essential for maintaining cardiac homeostasis.
  • Dysfunctional proteasomal activity is linked to the development of heart disease.
  • Cellular pathways involving proteasomal degradation are key to cardiovascular health.

Purpose of the Study:

  • To review the role of E3 ligases in heart biology.
  • To summarize how the proteasome regulates protein quality control in heart disease.
  • To discuss therapeutic strategies targeting the Ubiquitin Proteasome System in cardiovascular disease.

Main Methods:

  • Literature review of scientific publications.
  • Analysis of E3 ligase functions in cardiac tissue.
  • Examination of proteasomal pathways in cardiovascular disease models.

Main Results:

  • Identified specific E3 ligases critical for heart function.
  • Detailed the mechanisms of proteasomal protein degradation in cardiac pathology.
  • Highlighted the link between impaired proteasome function and heart disease progression.

Conclusions:

  • The Ubiquitin Proteasome System plays a vital role in cardiac homeostasis and disease.
  • Targeting E3 ligases and proteasomal pathways offers potential therapeutic avenues for heart conditions.
  • Further research into proteasome modulation could lead to novel treatments for cardiovascular diseases.

More Related Videos

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae09:05

Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

Published on: April 18, 2016

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones11:36

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

Published on: July 25, 2019

Related Concept Videos

The Proteasome01:13

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3 (ubiquitin...
The Proteasome02:18

The Proteasome

Eukaryotic cells can degrade proteins through several pathways. One of the most important amongst these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. A series of enzymes carry out the ubiquitination of the target proteins - E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation02:58

Regulated Protein Degradation

It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
The Proteasome Structure01:17

The Proteasome Structure

The ubiquitin-proteasome pathway is a well-known mechanism utilized by eukaryotic cells to remove cytoplasmic proteins that are misfolded, damaged, or no longer needed. In this pathway, the protein that needs to be eliminated undergoes a process called ubiquitination, where a chain of ubiquitin molecules is attached to the 48th lysine residue of the target protein. This ubiquitin modification helps the proteasome distinguish between a target protein and a healthy protein.
The proteasome is an...
Pathophysiology of Heart Failure01:17

Pathophysiology of Heart Failure

Heart failure (HF) is a progressive syndrome involving ventricles that leads to inadequate cardiac output. It can be classified based on location and output or ejection fraction. Ejection fraction (EF) is an essential measurement in the diagnosis and surveillance of HF. Reduced EF corresponds to systolic heart failure (HFrEF). However, HF with preserved ejection fraction (HFpEF) is becoming increasingly prevalent. Also known as diastolic HF, this form of HF is related to aging. The...