Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 12, 2026

Reusable Single Cell for Iterative Epigenomic Analyses
10:28

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

Simultaneously defining cell phenotypes, cell cycle, and chromatin modifications at single-cell resolution.

Allison B Chambliss1, Pei-Hsun Wu, Wei-Chiang Chen

  • 1Department of Chemical and Biomolecular Engineering, The Johns Hopkins University, 3400 N. Charles St., Baltimore, MD 21218, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|March 30, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CODAvision: best practices and a user-friendly interface for rapid, customizable segmentation of medical images.

Nature protocols·2026
Same author

The Emerging Role of Blood-Based Biomarkers in Predicting Alzheimer's Disease.

The journal of applied laboratory medicine·2026
Same author

Quantitative 3D histology reveals localized immune remodeling during early pancreatic cancer progression.

Cell press blue·2026
Same author

3D multi-omics tumour atlases: from technology to biology and clinical translation.

Nature reviews. Cancer·2026
Same author

Particle tracking microrheology of cancer cells in living subjects.

Materials today (Kidlington, England)·2026
Same author

Distinct senescent β-cell senotypes differentially drive islet aging and dysfunction.

bioRxiv : the preprint server for biology·2026

A new microscopy assay quantifies histone H3 acetylation and cell properties simultaneously at single-cell resolution. This method reveals how epigenetic variations predict phenotypic differences, even in cells with identical cell-cycle phases.

Area of Science:

  • Cell Biology
  • Epigenetics
  • Microscopy

Background:

  • Cellular heterogeneity arises from factors like cell cycle and chromatin modifications.
  • Existing methods lack simultaneous, in situ measurement of these properties at single-cell resolution.

Purpose of the Study:

  • To develop and validate a novel microscopy assay for simultaneous single-cell analysis.
  • To quantify global histone H3 acetylation alongside cell and nuclear properties.
  • To investigate the relationship between epigenetic modifications and cellular phenotypes.

Main Methods:

  • A new microscopy assay was developed and validated.
  • The assay quantifies histone H3 acetylation, cell-cycle phase, and F-actin content.
  • Thousands of cells were analyzed simultaneously in situ at single-cell resolution.
Keywords:
epigeneticshigh-throughputmicroscopy

More Related Videos

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Related Experiment Videos

Last Updated: May 12, 2026

Reusable Single Cell for Iterative Epigenomic Analyses
10:28

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease
09:20

Single-Cell Factor Localization on Chromatin using Ultra-Low Input Cleavage Under Targets and Release using Nuclease

Published on: February 1, 2022

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

Main Results:

  • The assay revealed significant variations in H3 acetylation among isogenic cells in the same cell-cycle phase.
  • H3 acetylation levels predicted variations in nuclear size and F-actin content.
  • Cellular properties were assessed across the cell cycle without synchronization artifacts.

Conclusions:

  • The developed assay enables simultaneous, high-resolution measurement of epigenetic modifications and cellular phenotypes.
  • Epigenetic heterogeneity contributes to phenotypic variation independently of cell-cycle phase.
  • The assay is versatile for studying drug effects on cell cycle, epigenetics, and phenotype.