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Related Concept Videos

MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometers01:16

Mass Spectrometers

This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
10:47

Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)

Published on: March 24, 2016

Mass spectrometry imaging for biomedical applications.

Jiangjiang Liu1, Zheng Ouyang

  • 1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

Analytical and Bioanalytical Chemistry
|March 30, 2013
PubMed
Summary

Mass spectrometry imaging (MSI) offers high-sensitivity tissue analysis. This review covers MSI technologies, challenges in 3D and quantitative imaging, and balancing resolution with biomedical applications.

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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization (IR-MALDESI)
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Published on: January 16, 2018

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Molecular Imaging

Background:

  • Mass spectrometry imaging (MSI) is a powerful technique for analyzing compound distribution in biological tissues with high sensitivity.
  • Traditional mass spectrometry (MS) methods require significant sample preparation and are often optimized for pharmaceutical analysis, limiting in-situ tissue imaging.
  • Developing MSI technologies is crucial for advancing in-situ biomedical research.

Purpose of the Study:

  • To critically review the current state of mass spectrometry imaging technologies.
  • To discuss the advancements and challenges in 3D and quantitative MSI.
  • To explore the importance of multi-modal imaging and the balance between high-resolution and practical biomedical applications.

Main Methods:

  • Review of current mass spectrometry imaging techniques.
  • Analysis of different sample ionization methods for MSI.
  • Discussion of 3D imaging, quantitative capabilities, and multi-modal approaches.

Main Results:

  • MSI provides highly specific and sensitive information on compound distribution in tissues.
  • In-situ tissue imaging requires adapted MS conditions and sample preparation.
  • Further development is needed for 3D and quantitative MSI capabilities.

Conclusions:

  • MSI holds significant potential for biomedical research, offering detailed molecular distribution data.
  • Optimizing MSI for in-situ analysis and addressing challenges in 3D/quantitative imaging are key research priorities.
  • Balancing high-resolution capabilities with practical biomedical applications is essential for widespread adoption of MSI.