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Related Experiment Video

Updated: May 12, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis

Published on: July 8, 2025

Quantitative detection of well-based DNA array using switchable lanthanide luminescence.

Ulla Karhunen¹, Minna Soikkeli, Susanne Lahdenperä

¹Division of Biotechnology, University of Turku, Turku, Finland. umskar@utu.fi

Analytica Chimica Acta

|April 2, 2013

Summary

A novel wash-free method uses switchable lanthanide luminescence for multiplexed DNA detection on solid-phase arrays. This technique enables sensitive and quantitative DNA analysis without requiring a washing step.

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Last Updated: May 12, 2026

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Area of Science:

Biotechnology
Analytical Chemistry
Molecular Diagnostics

Background:

Traditional DNA detection methods often involve multiple steps, including washing, which can be time-consuming and labor-intensive.
Developing rapid, sensitive, and wash-free assays is crucial for efficient molecular diagnostics and high-throughput screening.

Purpose of the Study:

To demonstrate a novel wash-free method for multiplexed DNA detection.
To employ switchable lanthanide luminescence technology on a solid-phase array for enhanced detection capabilities.
To achieve sensitive and quantitative DNA analysis with reduced assay complexity.

Main Methods:

Immobilization of oligonucleotide capture probes on a solid-phase array (microtiter plate well).
Hybridization of target nucleic acids with both capture and detection probes.
Self-assembly of non-luminescent lanthanide label molecules upon target binding to form a luminescent complex.
Time-resolved measurement of lanthanide luminescence directly from the solid-phase array without a wash step.

Main Results:

Quantitative detection of synthetic target oligonucleotides achieved with low detection limits (0.32 nM single target, 0.60 nM multiplexed assay).
Demonstrated qualitative detection of Polymerase Chain Reaction (PCR)-amplified targets from Escherichia coli.
The homogeneous, solid-phase array-based method proved effective for sensitive DNA analysis.

Conclusions:

The developed wash-free, lanthanide luminescence-based method offers a sensitive and efficient approach for multiplexed DNA detection.
This technology simplifies DNA analysis procedures by eliminating the need for washing steps.
The method shows promise for various applications in molecular diagnostics and biological research.