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Updated: May 12, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

Published on: April 16, 2016

Coffee ring aptasensor for rapid protein detection.

Jessica T Wen¹, Chih-Ming Ho, Peter B Lillehoj

¹Department of Bioengineering, University of California, Riverside, California 92521, United States.

Langmuir : the ACS Journal of Surfaces and Colloids

|April 2, 2013

Summary

This summary is machine-generated.

This study presents a novel biosensing platform using coffee ring effects for rapid protein detection. Highly hydrophobic surfaces enhance signal intensity sixfold, enabling sensitive biomarker detection in clinical samples.

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Last Updated: May 12, 2026

Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood

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Area of Science:

Biomolecular Engineering
Nanotechnology
Analytical Chemistry

Background:

Conventional protein detection methods often require complex instrumentation and trained personnel.
There is a need for simple, rapid, and cost-effective biosensing platforms for disease biomarker detection.

Purpose of the Study:

To develop and validate a novel biosensing platform for rapid protein detection.
To investigate the role of surface hydrophobicity in signal amplification using coffee ring formation.
To assess the platform's performance in detecting clinical biomarkers in biological fluids.

Main Methods:

Combination of coffee ring formation for biomolecular concentration and aptamer-based optical detection.
Utilized aptamer beacons for fluorescence signal transduction.
Investigated surface properties (hydrophobicity) for optimized concentration and signal amplification.

Main Results:

Coffee ring diameter is independent of surface hydrophobicity.
Highly hydrophobic surfaces enhanced particle concentration, yielding 6-fold greater signal intensity compared to hydrophilic surfaces.
Detection signals in serum and whole blood were up to 40 times higher than in liquid droplets.
Achieved a low limit of detection (2 ng/mL in serum, 4 ng/mL in blood) for α-thrombin.

Conclusions:

The developed biosensing platform offers a simple, sensitive, and cost-effective method for protein detection.
The coffee ring effect significantly amplifies signals, making it suitable for clinical diagnostics.
This platform holds potential for inexpensive disease biomarker detection, especially in resource-limited settings.