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Updated: May 12, 2026

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

A Novel Stretching Platform for Applications in Cell and Tissue Mechanobiology

Published on: June 3, 2014

Mechanical stretch changes coronary artery fibroblasts function by upregulating HSF1 protein expression.

Jiming Li¹, Yibo Zhang, Li Cui

¹Department of Cardiology, East Hospital, Tongji University School of Medicine, Shanghai 200120, China. lxb70@hotmail.com

International Journal of Biological Macromolecules

|April 2, 2013

Summary

Mechanical stretch enhances fibroblast proliferation and collagen production. This process may involve the upregulation of Heat Shock Factor 1 (HSF1) expression, influencing cellular function and inflammatory responses.

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Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch

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Published on: April 21, 2023

Area of Science:

Cardiovascular Biology
Cellular Mechanotransduction

Background:

Fibroblast cells play a crucial role in tissue repair and remodeling.
Mechanical forces are increasingly recognized as significant regulators of cellular behavior in cardiovascular tissues.

Purpose of the Study:

To investigate the impact of mechanical stretch on human coronary artery fibroblast function.
To elucidate the molecular mechanisms underlying these changes, particularly the role of HSF1.

Main Methods:

Cell culture of human coronary artery fibroblasts.
Application of mechanical stretch (24h) to a subset of cells.
Assays for proliferation (CCK-8), collagen secretion (ELISA, Hydroxyproline), and gene expression (RT-PCR) of MMP, TIMP, IL-6, alpha-SMA, HSF1, HSP70.
Protein expression analysis of HSF1 (Western-blotting).

Main Results:

Mechanical stretch significantly increased fibroblast proliferation and collagen secretion.
Upregulation of MMP-9/TIMP ratio, alpha-SMA, IL-6, HSF1, and HSP70 mRNA was observed in the stretch group.
Western-blotting confirmed increased HSF1 protein expression under mechanical stretch.

Conclusions:

Mechanical stretch alters human coronary artery fibroblast proliferation and collagen formation.
The observed changes in cellular function and inflammatory factor secretion are potentially mediated by the upregulation of HSF1 protein expression.