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Updated: May 12, 2026

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

Quantitative cross-linking/mass spectrometry using isotope-labelled cross-linkers

Lutz Fischer1, Zhuo Angel Chen, Juri Rappsilber

  • 1Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, United Kingdom.

Journal of Proteomics
|April 2, 2013
PubMed
Summary

Quantitative cross-linking/mass spectrometry (CLMS) is now feasible for studying dynamic protein complexes. We developed a new strategy and open-source software (XiQ) to overcome limitations in quantifying cross-linked proteins.

Keywords:
Cross-linkingMass spectrometryProtein dynamicsProteomicsQuantitationStructural biology

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