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Tissue Homogenization and Cell Lysis

Tissue Homogenization and Cell Lysis

Tissue homogenization involves disintegrating tissue architecture and lysing cells, and is an early step in isolating and analyzing cellular components. The method used for homogenization depends on the sample type, the amount of sample available, the analyte to be obtained, and the sensitivity of the method. These methods are broadly classified as mechanical and non-mechanical methods.
Mechanical methods of tissue homogenization
These methods rely on applying external physical force to disrupt...

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Related Experiment Video

Updated: May 12, 2026

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

Published on: December 12, 2016

A simple and fast method for tissue cryohomogenization enabling multifarious molecular extraction.

Lukas M von Ziegler¹, Bechara J Saab, Isabelle M Mansuy

¹Brain Research Institute, Faculty of Medicine, University of Zürich, Zürich, Switzerland.

Journal of Neuroscience Methods

|April 2, 2013

Summary

This summary is machine-generated.

This study refines cryohomogenization for molecular biology, enabling high-quality RNA and protein extraction from single tissue samples. This method optimizes parallel molecular analysis, reducing operational and ethical burdens.

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Last Updated: May 12, 2026

Cryosectioning of Contiguous Regions of a Single Mouse Skeletal Muscle for Gene Expression and Histological Analyses

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Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

Preparation of Cell Extracts by Cryogrinding in an Automated Freezer Mill

Published on: January 29, 2021

Area of Science:

Molecular Biology
Neuroscience
Biochemistry

Background:

Parallel analysis of nucleic acids and proteins from single tissues is crucial in molecular biology.
Current methods often compromise sample quality or require multiple specimens, posing operational and ethical challenges.

Purpose of the Study:

To refine cryohomogenization techniques for efficient parallel molecular extraction from single tissue samples.
To enable immediate or post-storage fractionation for diverse molecular component analysis.

Main Methods:

Cryohomogenization of tissue samples.
Fractionation of homogenized samples for molecular component extraction.
Analysis of RNA and protein quality and quantity using western blot, nanodrop UV/V spectrometry, and bioanalyzer.

Main Results:

Cryohomogenization yields high-quality RNA and protein from hippocampus samples.
No significant loss in molecular abundance was observed post-cryohomogenization.
The method is suitable for both immediate use and storage of samples.

Conclusions:

Refined cryohomogenization allows efficient parallel molecular extraction from single tissue samples.
This technique preserves molecular integrity, offering an optimal solution for operational and ethical considerations.
Particularly beneficial for analyzing laterally organized brain structures like the hippocampus.