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Updated: May 12, 2026

A Simple Method of Mouse Lung Intubation

A Simple Method of Mouse Lung Intubation

Published on: March 21, 2013

A simple method of mouse lung intubation.

Sandhya Das¹, Kelvin MacDonald, Herng-Yu Sucie Chang

¹Department of Environmental Health Sciences, Program in Respiratory Biology and Lung Disease, Johns Hopkins Bloomberg School of Public Health.

Journal of Visualized Experiments : Jove

|April 2, 2013

Summary

A novel fiberoptic technique simplifies mouse intubation for pulmonary function testing. This method enables longitudinal lung mechanics studies, reducing animal numbers and enhancing statistical power.

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Area of Science:

Physiology
Animal Models
Respiratory Research

Background:

Longitudinal pulmonary function measurements in mice are valuable for research but challenging due to difficult intubation.
Existing intubation methods often lack consistent tracheal visualization, hindering routine application.

Purpose of the Study:

To present a simplified, cost-effective fiberoptic-guided intubation procedure for mice.
To enable reliable serial pulmonary function measurements in individual mice.

Main Methods:

Utilized a 0.5 mm fiberoptic light source as a guide for intubation cannula insertion into the mouse trachea.
Demonstrated the procedure's efficacy in measuring lung mechanics over several weeks.

Main Results:

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Last Updated: May 12, 2026

A Simple Method of Mouse Lung Intubation

A Simple Method of Mouse Lung Intubation

Published on: March 21, 2013

Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung

Noninvasive Intratracheal Intubation to Study the Pathology and Physiology of Mouse Lung

Published on: November 8, 2013

Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope

Endotracheal Intubation in Mice via Direct Laryngoscopy Using an Otoscope

Published on: April 5, 2014

The fiberoptic guidance significantly improved tracheal visualization during intubation.
Lung mechanics were successfully measured repeatedly in individual mice for extended periods (weeks).
The technique requires minimal expense, expertise, and training for routine use.

Conclusions:

This fiberoptic-guided intubation method provides a practical solution for longitudinal pulmonary studies in mice.
The procedure minimizes the number of animals required and increases statistical power by enabling within-subject comparisons.