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Related Experiment Video

Updated: May 12, 2026

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
07:23

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Published on: July 16, 2010

Using zebrafish transgenesis to test human genomic sequences for specific enhancer activity.

Minaka Ishibashi1, Alejandro S Mechaly, Thomas S Becker

  • 1Brain and Mind Research Institute, Sydney Medical School, University of Sydney, 100 Mallet Street, Camperdown 2050, Australia.

Methods (San Diego, Calif.)
|April 2, 2013
PubMed
Summary

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This study presents a new method to find human tissue-specific transcriptional enhancers using computational analysis and zebrafish reporter assays. The approach successfully identifies and validates functional enhancers for gene regulation studies.

Area of Science:

  • Genomics
  • Molecular Biology
  • Developmental Biology

Background:

  • Identifying tissue-specific transcriptional enhancers is crucial for understanding gene regulation.
  • Existing methods may lack efficiency or comprehensive validation for enhancer discovery.

Purpose of the Study:

  • To develop and validate a robust, multi-step approach for identifying human tissue-specific transcriptional enhancers.
  • To leverage computational predictions and a transgenic zebrafish model for functional enhancer testing.

Main Methods:

  • Defined search space around target genes for candidate enhancer identification.
  • Utilized evolutionary conservation, transcription factor binding, and chromatin marks (e.g., ENCODE data) for in silico candidate selection.
  • Employed a Tol2 transposon-based zebrafish reporter assay for functional validation of candidate enhancers.
Keywords:
EnhancerGene regulationHuman genomeMammalian genomeReporter gene expressionVertebrate genomeZebrafish transgenesis

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Last Updated: May 12, 2026

Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences
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Mosaic Zebrafish Transgenesis for Evaluating Enhancer Sequences

Published on: July 16, 2010

Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis
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Mosaic Zebrafish Transgenesis for Functional Genomic Analysis of Candidate Cooperative Genes in Tumor Pathogenesis

Published on: March 31, 2015

Gene Trapping Using Gal4 in Zebrafish
13:34

Gene Trapping Using Gal4 in Zebrafish

Published on: September 29, 2013

Main Results:

  • Successfully identified and validated human tissue-specific enhancers through genomic insertions in zebrafish.
  • Demonstrated the reproducibility and tissue specificity of identified enhancers in transgenic offspring.

Conclusions:

  • The developed approach provides an effective strategy for discovering functional human transcriptional enhancers.
  • This method facilitates the study of gene regulation in specific tissues and developmental contexts.