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Development of a reverse transcription-quantitative PCR assay for detection of salivirus/klassevirus

Eiji Haramoto1, Masaaki Kitajima, Mikie Otagiri

  • 1International Research Center for River Basin Environment, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Yamanashi, Japan. eharamoto@yamanashi.ac.jp

Applied and Environmental Microbiology
|April 2, 2013
PubMed

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Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...