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Updated: May 12, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Sphingobium sufflavum sp. nov., isolated from a freshwater lake
Shih-Yi Sheu1, Yu-Wen Shiau1, Wen-Ming Chen2
1Department of Marine Biotechnology, National Kaohsiung Marine University, No. 142, Hai-Chuan Rd. Nan-Tzu, Kaohsiung City 811, Taiwan, ROC.
Abstract:
A Gram-stain negative, rod-shaped, non-motile, non-spore-forming and aerobic bacterial strain, designated HL-25(T), was isolated and characterized in a taxonomic study using a polyphasic approach. Comparative analysis of the 16S rRNA gene sequences showed that the isolate constituted a distinct branch within the genus Sphingobium, showing the highest level of 16S rRNA gene sequence similarity to Sphingobium vulgare HU1-GD12(T) (96.6 %). The major fatty acids (>10%) of strain HL-25(T) were C(18:1)ω7c, C(16:0), summed feature 3 (comprising C(16:1)ω7c and/or C(16:1)ω6c) and C(18:0). The major cellular hydroxy fatty acid was C(14:0) 2-OH. The major isoprenoid quinone was Q-10 and the DNA G+C content was 63.8 mol%. The polar lipid profile consisted of a mixture of sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, phosphatidyldimethylethanolamine, diphosphatidylglycerol, an uncharacterized glycolipid, an uncharacterized aminophospholipid and four uncharacterized phospholipids. The polyamine pattern of strain HL-25(T) contained spermidine and putrescine. On the basis of these genotypic, chemotaxonomic and phenotypic data, strain HL-25(T) represents a novel species in the genus Sphingobium, for which the name Sphingobium sufflavum sp. nov. is proposed. The type strain is HL-25(T) ( =BCRC 80413(T) =KCTC 23953(T)).
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