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Updated: May 12, 2026

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
Published on: January 9, 2017
Aspects of determining the molecular weight of cyclodextrin polymers and oligomers by static light scattering
István Puskás1, Alexandra Szemjonov, Éva Fenyvesi
1CycloLab Research and Development Laboratory Ltd., 1097 Budapest, Illatos út 7, Hungary. puskas@cyclolab.hu
Abstract:
Methodic issues affecting the use of static light scattering to determine the average molecular weight of cyclodextrin poly- and oligomers are discussed. The critical features which enable accurate measurement such as aggregation behavior and segment-segment interaction are elucidated. Static light scattering data supported with globule size and aggregate state analysis (as determined by dynamic light scattering) allowed the aggregate-free state of the samples to be justified and the ideal globular conformation of the macromolecules to be corroborated. These molecular characteristics were demonstrated for uncharged, charged and fluorescent randomly crosslinked water soluble cyclodextrin poly- and oligomers.
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