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A SRCF cell line from snowtrout, Schizothorax richardsonii: development and characterization
M Goswami1, B S Sharma, S N Bahuguna
1National Bureau of Fish Genetic Resources, Canal Ring Road, PO Dilkusha, Lucknow 226002, India. mukugoswami@gmail.com
Tissue & Cell
|April 3, 2013
Summary
A new cell line, SRCF, was developed from snowtrout (Schizothorax richardsonii), a valuable Himalayan fish. This SRCF cell line shows potential for genetic research and aquatic toxicology studies.
Area of Science:
- Aquaculture
- Fish cell line development
- Molecular biology
Background:
- Schizothorax richardsonii (snowtrout) is a commercially important coldwater fish native to the Himalayas.
- There is a need for established cell lines for research on indigenous fish species.
Purpose of the Study:
- To develop and characterize a novel cell line from Schizothorax richardsonii.
- To evaluate the potential applications of this cell line in genetic improvement and toxicology.
Main Methods:
- Cell culture from caudal fin tissue using Leibovitz's L-15 medium with 10% FBS at 24°C.
- Morphological, chromosomal, and mitochondrial DNA (mt-DNA) analysis (COI and 16S rRNA genes).
- Transfection with pEGFP-C1 plasmid and genotoxicity assessment.
Main Results:
- A fibroblastic cell line (SRCF) was successfully established and maintained.
- The cell line exhibited a diploid chromosome count of 98 and confirmed origin via mt-DNA sequencing.
- Successful transfection and positive results in genotoxicity assays were observed.
Conclusions:
- The SRCF cell line is a valuable new resource for Schizothorax richardsonii research.
- This cell line is suitable for transgenic applications and as an in vitro model for aquatic toxicology.
- The established cell line supports genetic improvement programs for this important fish species.
