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Preventing adsorption of immunoglobulin G to solid surfaces using poloxamer 407 eliminates artifactual stimulation of

Iwan Kustiawan1, Ninotska I L Derksen, Theo Rispens

  • 1Sanquin Research and Landsteiner Laboratory, Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands. I.Kustiawan@Sanquin.nl

Journal of Immunological Methods
|April 3, 2013
PubMed

To study the effect of polyclonal intravenous immunoglobulin G (IVIG) on neutrophils in vitro, adsorption of immunoglobulin G (IgG) to solid surfaces has to be prevented, because IgG bound to a solid surface can activate neutrophils through activating FcγRs. In this study we demonstrate that poloxamer 407, a non ionic surfactant, at low concentration (0.05%) prevented the adsorption of high concentrations of IgG (5 mg/ml) better than other blocking agents without interfering with the interaction of IgG with the neutrophils. Poloxamer 407 is therefore a suitable blocking agent to prevent the interaction of immunoglobulin with solid surfaces in cell-based in vitro experiments.

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