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Related Concept Videos

Chemical Agents for Microbial Control01:27

Chemical Agents for Microbial Control

Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
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Biological Methods for Microbial Control

Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
Microbial Corrosion01:24

Microbial Corrosion

Microbiologically Influenced Corrosion (MIC) is a significant form of material degradation caused by the metabolic activities of microorganisms. This phenomenon poses substantial challenges across various industries, including oil and gas, maritime, and water treatment sectors.MIC occurs when microorganisms, such as bacteria, archaea, and fungi, colonize metal surfaces, forming biofilms that alter the local electrochemical environment. These biofilms can lead to the production of corrosive...

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Related Experiment Video

Updated: May 12, 2026

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
11:19

Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties

Published on: May 10, 2018

Enzyme-based listericidal nanocomposites.

Kusum Solanki1, Navdeep Grover, Patrick Downs

  • 1Department of Chemical and Biological Engineering, Center for Biotechnology & Interdisciplinary Studies, and Rensselaer Nanotechnology Center, Rensselaer Polytechnic Institute, Troy, NY 12180, USA.

Scientific Reports
|April 3, 2013
PubMed
Summary
This summary is machine-generated.

Researchers developed novel surface-incorporated Ply500 enzymes to combat Listeria. These formulations effectively kill pathogenic bacteria on surfaces, offering a new decontamination strategy.

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Synthesis of Multi-walled Carbon Nanotubes Modified with Silver Nanoparticles and Evaluation of Their Antibacterial Activities and Cytotoxic Properties
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Published on: May 10, 2018

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

Area of Science:

  • Biotechnology
  • Food Safety
  • Microbiology

Background:

  • Cell lytic enzymes offer an alternative to antibiotics for killing pathogenic bacteria like Listeria.
  • Phage-derived lytic enzymes show promise, but their surface incorporation for decontamination is unexplored.

Purpose of the Study:

  • To develop and evaluate surface-incorporated Ply500 endolysin formulations for Listeria decontamination.
  • To explore facile methods for attaching Ply500 to various nanoparticle surfaces.

Main Methods:

  • Covalent attachment of Ply500 to silica nanoparticles (SNPs).
  • Incorporation of SNP-Ply500 conjugates into poly(hydroxyethyl methacrylate) films.
  • Affinity binding of Ply500 to starch nanoparticles using a maltose binding protein fusion.

Main Results:

  • All three Ply500 formulations demonstrated effective killing of Listeria innocua.
  • Antimicrobial activity was confirmed in both PBS and on lettuce surfaces.
  • Successful surface incorporation of the listeria bacteriophage endolysin was achieved.

Conclusions:

  • Surface-incorporated Ply500 formulations provide an effective strategy for pathogen decontamination.
  • This approach offers a novel route for selective and efficient pathogen control in public infrastructure.
  • The developed methods enable the application of lytic enzymes for enhanced food safety and surface disinfection.