Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Novel Bioluminescence Tomography System Compatible with CBCT-guided Small Animal Irradiators for High-Precision Preclinical Radiation Research.

Radiation research·2026
Same author

Simulating light transmission and wavefront shaping in turbid media with a T-matrix method.

Optics express·2025
Same author

Feasibility of diffuse Raman spectroscopy to detect in-vivo molecular changes in the tissue induced by subcutaneous implants.

Biomedical optics express·2025
Same author

Assessment of a VoIP steganalysis method based on statistical analysis and deep neural network.

Scientific reports·2025
Same author

High precision fluorescence tomography system for pre-clinical radiation research: system design and validation.

Physics in medicine and biology·2025
Same author

Sensitive broadband ultrasound sensor based on a low-loss high-Q fused-silica plano-concave microresonator.

Optics express·2025
Same journal

Denoising algorithm of Φ-OTDR systems based on adaptive fractional wavelet transform denoising.

Optics express·2026
Same journal

Millisecond photon-to-photon latency and high-speed volumetric projection system for optogenetics.

Optics express·2026
Same journal

Polarization-encoded coaxial structured light for high-precision 3D surface profilometry.

Optics express·2026
Same journal

Discrete freeform optical design based on collaborative optimization of point cloud and local normals.

Optics express·2026
Same journal

Ultrafast ghost imaging with 25 GHz speckle switching and wavelength-division multiplexing.

Optics express·2026
Same journal

Atomic vapor cells fabricated by femtosecond laser welding of standard-optical-quality glass.

Optics express·2026
See all related articles

Related Experiment Video

Updated: May 12, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

Simultaneous multiple view high resolution surface geometry acquisition using structured light and mirrors.

Hector R A Basevi1, James A Guggenheim, Hamid Dehghani

  • 1PSIBS, University of Birmingham, Edgbaston, Birmingham, B15 2TT, United Kingdom. hrb886@bham.ac.uk

Optics Express
|April 3, 2013
PubMed
Summary
This summary is machine-generated.

A new, inexpensive fringe projection profilometry system captures 3D surface geometry using multiple views and mirrors. This advanced imaging technique achieves high accuracy for reconstructing 3D models from complex subjects.

More Related Videos

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
07:27

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

Related Experiment Videos

Last Updated: May 12, 2026

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors
11:15

A Guide to Structured Illumination TIRF Microscopy at High Speed with Multiple Colors

Published on: May 30, 2016

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques
11:34

High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

Published on: December 3, 2013

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
07:27

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Published on: November 21, 2016

Area of Science:

  • Optical imaging
  • 3D reconstruction
  • Metrology

Background:

  • Accurate surface geometry is crucial for various imaging applications.
  • Existing 3D surface imaging techniques face limitations like restricted geometry, high costs, and mechanical complexity.
  • Fringe projection profilometry is a viable method for surface geometry acquisition.

Purpose of the Study:

  • To develop an affordable, generalized fringe projection profilometry system.
  • To overcome limitations of existing systems by allowing arbitrary component placement and using mirrors for expanded field of view.
  • To enable simultaneous multi-view 3D surface data acquisition.

Main Methods:

  • Proposed an inexpensive fringe projection profilometry system adaptable to arbitrary component placement.
  • Incorporated mirrors to enhance the field of view.
  • Simultaneously acquired multiple views of imaging subjects.
  • Integrated the prototype into a Diffuse Optical Tomography and Bioluminescence Tomography small animal imaging system.
  • Processed point clouds to generate 3D surface meshes.

Main Results:

  • The system successfully imaged various objects, including a mouse phantom, mouse cadaver, and coin.
  • A prototype demonstrated high accuracy, with 50% of points within 0.1mm, 82% within 0.2mm, and 96% within 0.4mm of the ideal surface for a mouse phantom.
  • The generated surface mesh showed excellent agreement with manufacturer-provided ideal surface points.

Conclusions:

  • The developed fringe projection profilometry system offers an inexpensive and versatile solution for 3D surface geometry acquisition.
  • The system's ability to handle arbitrary configurations and expand field of view makes it suitable for diverse applications, including small animal imaging.
  • The achieved accuracy validates the system's potential for generating precise 3D models.