Related Experiment Video
Updated: May 12, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Tomographic imaging via spectral encoding of spatial frequency
Shikhar Uttam1, Sergey A Alexandrov, Rajan K Bista
1Biomedical and Optical Imaging Laboratory (BOIL), Departments of Medicine and Bioengineering, University of Pittsburgh, Pittsburgh PA 15213, USA. shf28@pitt.edu
Abstract:
Three-dimensional optical tomographic imaging plays an important role in biomedical research and clinical applications. We introduce spectral tomographic imaging (STI) via spectral encoding of spatial frequency principle that not only has the capability for visualizing the three-dimensional object at sub-micron resolution but also providing spatially-resolved quantitative characterization of its structure with nanoscale accuracy for any volume of interest within the object. The theoretical basis and the proof-of-concept numerical simulations are presented to demonstrate the feasibility of spectral tomographic imaging.
Related Concept Videos
Computed Tomography
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Imaging Studies III: Computed Tomography
Continuous -time Fourier Transform
Imaging Studies I: CT and MRI
Description of the Procedures
Computed Tomography (CT) scan:
Computed Tomography (CT) scans use X-ray technology to generate detailed images of bones, organs, and tissues. During the scan, the patient lies on a moving table...
Aliasing
If the sampling frequency is below the Nyquist rate, these replicas overlap, preventing the original signal...
