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Multidentate zwitterionic chitosan oligosaccharide modified gold nanoparticles: stability, biocompatibility and cell
Xiangsheng Liu1, Haoyuan Huang, Gongyan Liu
1MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Nanoscale
|April 3, 2013
Summary
Novel zwitterionic biopolymers enhance gold nanoparticle (AuNP) stability and biocompatibility. These engineered AuNPs show excellent performance in extreme conditions and low cytotoxicity, improving nanoparticle applications in biosystems.
Area of Science:
- Nanotechnology
- Biomaterials Science
- Surface Chemistry
Background:
- Surface engineering of nanoparticles is crucial for their stability and biological interactions.
- Chitosan oligosaccharides offer a versatile backbone for functionalization.
Purpose of the Study:
- To develop a novel multidentate zwitterionic biopolymer for nanoparticle surface modification.
- To evaluate the colloidal stability, biocompatibility, and intracellular fate of modified gold nanoparticles (AuNPs).
Main Methods:
- Conjugation of lipoic acid and acryloyloxyethyl phosphorylcholine to chitosan oligosaccharide.
- Characterization of modified AuNPs' stability under extreme conditions (high salt, pH, serum).
- Assessment of cytotoxicity (MTT, LDH assays) and hemocompatibility (hemolysis assay).
- Investigation of intracellular fate using ICP-MS and TEM.
Main Results:
- Modified AuNPs exhibited remarkable colloidal stability in high salt, wide pH range, and serum/plasma media.
- High resistance to dithiothreitol competition and low cytotoxicity were observed.
- Good hemocompatibility and cellular uptake in a concentration-dependent manner were demonstrated.
- Endosomal escape and nuclear accumulation of AuNPs were observed within 24 hours.
Conclusions:
- The novel multidentate zwitterionic biopolymer provides superior stabilization for gold nanoparticles.
- Modified AuNPs show excellent biocompatibility and predictable intracellular behavior.
- This polymer derivative holds potential for stabilizing various inorganic nanoparticles for bio-related applications.
