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Updated: May 12, 2026

Direct Protein Delivery to Mammalian Cells Using Cell-permeable Cys2-His2 Zinc-finger Domains
Published on: March 25, 2015
Intracellular delivery of biologically active proteins with peptide-based carriers
1Department of Biological Sciences, National University of Singapore, Singapore, Singapore.
Abstract:
The medical applications of protein-based therapeutics are hampered by low bioavailability associated with inefficient intracellular delivery. Various delivery materials have been developed and tested to interact with protein cargos in a manner of stabilizing proteins extracellularly and facilitating cellular uptake of proteins, thus enhancing delivery efficiency. Peptides that can form stable complexes with proteins through non-covalent interaction appear to be a promising tool to improve intracellular delivery of proteins. Here we describe the preparation of complexes formed between β-galactosidase and peptide-based carrier, protein transfer of the complexes, and the methods to evaluate delivery efficiency qualitatively and quantitatively.
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