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Updated: May 12, 2026

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Fluorescent spherical monodisperse silica core-shell nanoparticles with a protein-binding biofunctional shell.

Achim Weber1, Marion Herz, Günter E M Tovar

  • 1Fraunhofer Institute for Interfacial Engineering and Biotechnology IGB, Stuttgart, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|April 3, 2013
PubMed
Summary

Uniform fluorescent silica nanoparticles with protein-binding capabilities were created using a modified Stöber method. These biofunctional nanoparticles can serve as advanced imaging tools and reporter systems for molecular recognition.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biotechnology

Background:

  • Development of functional nanoparticles for biological applications is crucial.
  • Protein-conjugated nanoparticles offer potential for molecular imaging and diagnostics.
  • Controlled synthesis of uniform core-shell nanostructures is challenging.

Purpose of the Study:

  • To synthesize uniform, fluorescent, protein-binding silica core-shell nanoparticles.
  • To functionalize nanoparticle surfaces for efficient protein conjugation.
  • To characterize the synthesized nanoparticles and their protein attachment.

Main Methods:

  • Modified Stöber method for synthesizing 100 nm fluorescent silica cores.
  • Generation of organic shells with amine and carboxyl groups for functionalization.

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  • Protein conjugation using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC).
  • Characterization via microelectrophoresis, dynamic light scattering (DLS), and bicinchoninic acid (BCA) assay.

    • Main Results:

    • Successful production of uniform fluorescent silica core-shell nanoparticles.
    • Demonstrated surface functionalization with amine and carboxyl groups.
    • Quantified protein conjugation using EDC coupling and BCA assay.
    • Characterization confirmed nanoparticle size, surface properties, and protein attachment.

    Conclusions:

    • The modified Stöber method enables the synthesis of uniform, biofunctional fluorescent silica nanoparticles.
    • These nanoparticles are suitable for protein conjugation, creating effective molecular recognition systems.
    • The developed nanoparticles hold promise as imaging agents and reporter systems in biological applications.