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Related Concept Videos

Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...

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Related Experiment Video

Updated: May 12, 2026

Purification of Platelets from Mouse Blood
05:41

Purification of Platelets from Mouse Blood

Published on: May 7, 2019

Platelet counting.

Sylvain Salignac1, Véronique Latger-Cannard, Nicole Schlegel

  • 1Hematology Department and Grand East Competence Center on Inherited Platelet Disorders, CHU Nancy, Nancy, France.

Methods in Molecular Biology (Clifton, N.J.)
|April 3, 2013
PubMed
Summary
This summary is machine-generated.

Automated and flow cytometry methods offer faster, more precise platelet counting than manual microscopy. Adhering to pre-analytical and analytical variables is essential for reliable hematological analysis results.

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Related Experiment Videos

Last Updated: May 12, 2026

Purification of Platelets from Mouse Blood
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Published on: May 7, 2019

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
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Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies
07:06

Preparation of Washed Human Platelets for Quantitative Metabolic Flux Studies

Published on: January 10, 2025

Area of Science:

  • Hematology
  • Clinical Diagnostics
  • Flow Cytometry

Background:

  • Platelet counting is a fundamental daily hematological analysis with significant clinical implications.
  • Manual microscopy techniques have largely been superseded by automated methods for speed and precision.
  • Flow cytometry is emerging as a reference standard for platelet enumeration.

Purpose of the Study:

  • To review the evolution and current state of platelet counting methodologies.
  • To highlight the importance of pre-analytical and analytical variables in ensuring reliable results.
  • To discuss the transition from manual to automated and advanced techniques.

Main Methods:

  • Comparison of historical manual techniques (phase-contrast microscopy) with automated analyzers (impedance, optical).
  • Evaluation of flow cytometry methods utilizing labeled monoclonal antibodies.
  • Emphasis on the critical role of pre-analytical and analytical phase controls.

Main Results:

  • Automated techniques provide enhanced rapidity and precision over manual methods.
  • Flow cytometry presents a promising reference technique for platelet counting.
  • Strict adherence to procedural variables is paramount for result validity.

Conclusions:

  • The field of platelet counting has advanced significantly with automated and flow cytometry approaches.
  • Maintaining rigorous standards in pre-analytical and analytical phases is crucial for accurate clinical decision-making.
  • Understanding these methodological shifts is key to avoiding validation pitfalls in hematology labs.