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Related Experiment Video

Updated: May 12, 2026

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
12:13

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Stable transfection using episomal vectors to create modified human embryonic stem cells.

Ying Liu1, Kate Judd, Uma Lakshmipathy

  • 1Department of Neurosurgery, UT Health, Houston, TX, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 3, 2013
PubMed
Summary

Episomal vectors offer a nonviral gene delivery method for human embryonic stem cells, avoiding genomic integration for stable transgene expression. This study details their transfection and clone creation.

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Area of Science:

  • Stem cell biology
  • Molecular biology
  • Gene therapy

Background:

  • Gene delivery to stem cells is crucial for research and therapeutic applications.
  • Viral vectors are common but raise safety concerns.
  • Nonviral methods, particularly episomal vectors, offer an alternative that avoids genomic integration.

Purpose of the Study:

  • To detail the process of transfecting human embryonic stem cells (hESCs) with episomal vectors.
  • To describe the creation of stable, pooled hESC clones using these nonintegrating vectors.

Main Methods:

  • Utilized nonviral gene delivery using episomal vectors.
  • Performed transfection of human embryonic stem cells.
  • Established stable pooled clones expressing transgenes from episomal DNA.

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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
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Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Published on: October 5, 2011

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
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Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Published on: October 28, 2014

Related Experiment Videos

Last Updated: May 12, 2026

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
12:13

Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells

Published on: August 23, 2014

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
10:24

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Published on: October 5, 2011

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
08:14

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Published on: October 28, 2014

Main Results:

  • Successfully transfected hESCs with episomal vectors.
  • Generated stable pooled clones of hESCs maintaining episomal transgene expression.
  • Demonstrated transgene expression independent of genomic integration.

Conclusions:

  • Episomal vectors provide a viable nonviral strategy for gene delivery in hESCs.
  • This method allows for transgene expression without the risks associated with genomic integration.
  • The described protocol facilitates the creation of genetically modified hESC lines for research.