Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...

Electro-mechanical Systems

Electro-mechanical Systems

Electromechanical systems are intricate configurations that effectively combine electrical and mechanical elements to achieve a desired outcome. Central to many of these systems is the DC motor, a device that converts electrical energy into mechanical motion, enabling various applications ranging from simple fans to complex robotic mechanisms.
A key component of the DC motor is the armature, a rotating circuit positioned within a magnetic field. As an electric current passes through the...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Development of Standardized Methods and Analytical Tools.

Advances in experimental medicine and biology·2025

Same author

Generation and comprehensive characterization of induced pluripotent stem cells for translational research.

Regenerative medicine·2019

Same author

Generation of induced pluripotent stem cells with CytoTune, a non-integrating Sendai virus.

Methods in molecular biology (Clifton, N.J.)·2013

Same author

Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined Essential 8™ Medium conditions.

Methods in molecular biology (Clifton, N.J.)·2013

Same author

Generation of human-induced pluripotent stem cells by a nonintegrating RNA Sendai virus vector in feeder-free or xeno-free conditions.

Stem cells international·2012

Same author

Chromatin insulator elements block transgene silencing in engineered human embryonic stem cell lines at a defined chromosome 13 locus.

Stem cells and development·2011

Search research articles

Related Experiment Video

Updated: May 12, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

Site-specific integration in human ESC using Jump-In™ TI™ technology.

Chad C Macarthur¹

¹Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 3, 2013

Summary

Jump-In™ TI™ technology uses bacteriophage recombinases to precisely engineer human embryonic stem cells (hESCs). This method ensures stable, single-copy gene integration for efficient study of gene function in pluripotent and differentiated cells.

More Related Videos

Early Metamorphic Insertion Technology for Insect Flight Behavior Monitoring

Early Metamorphic Insertion Technology for Insect Flight Behavior Monitoring

Published on: July 12, 2014

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Related Experiment Videos

Last Updated: May 12, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

Early Metamorphic Insertion Technology for Insect Flight Behavior Monitoring

Early Metamorphic Insertion Technology for Insect Flight Behavior Monitoring

Published on: July 12, 2014

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Non-Viral Engineering of Primary Human T Cells via Homology-Mediated End-Joining Targeted Integration of Large DNA Templates

Published on: May 9, 2025

Area of Science:

Stem cell biology
Molecular biology
Genetic engineering

Background:

Engineering human embryonic stem cells (hESCs) is crucial for studying human gene function.
Existing methods for engineering hESCs often lack specificity or efficiency.
Stable, targeted genetic modification is needed for reliable research.

Purpose of the Study:

To present a novel technology for specific and efficient genetic engineering of hESCs.
To demonstrate the stable, single-copy integration of genetic elements into the hESC genome.
To evaluate the expression of integrated genes in pluripotent and differentiated cells.

Main Methods:

Utilized Jump-In™ TI™ technology employing PhiC31 and R4 bacteriophage recombinases.
Introduced a targeting site to a defined genomic locus in hESCs.
Delivered genetic elements of interest to the targeting site for integration.

Main Results:

Achieved specific and efficient integration of genetic elements into the hESC genome.
Demonstrated stable, single-copy integration at a defined locus.
Observed high expression levels of integrated elements in both pluripotent and differentiated cells.

Conclusions:

Jump-In™ TI™ technology provides a robust platform for engineering hESCs.
This method enables precise and stable genetic modification for gene function studies.
The technology supports consistent gene expression across various cell states.