Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Climate Transformation and Stewardship: Reflections on Meaningful Collaboration to Support Indigenous-Led Research.

Ecology and evolution·2025
Same author

Phenological Variation and Evolution Across Space and Time in the Scarlet Monkeyflower (<i>Erythranthe cardinalis</i>).

Ecology and evolution·2025
Same author

Evolutionary Responses to Historic Drought across the Range of Scarlet Monkeyflower.

The American naturalist·2025
Same author

Prickly Problems: <i>Cylindropuntia</i>'s Low Genetic Diversity Despite Inbreeding Avoidance.

Ecology and evolution·2025
Same author

Influence of sexual dimorphism and dichromatism on reproductive success in a rare native cactus.

Oecologia·2023
Same author

Sequential hybridization may have facilitated ecological transitions in the Southwestern pinyon pine syngameon.

The New phytologist·2022

Related Experiment Video

Updated: May 12, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

Scoring microsatellite loci.

Lluvia Flores-Rentería1, Andrew Krohn

  • 1Department of Biological Sciences, Merriam-Powell Center for Environmental Research, Northern Arizona University, Flagstaff, AZ, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 3, 2013
PubMed
Summary

Automated microsatellite genotyping is fast but prone to errors. Understanding potential pitfalls from DNA extraction to data entry is crucial for accurate sample genotyping and reliable research conclusions.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Microsatellites are widely used for individual genotyping in diverse research fields.
  • Automated scoring software accelerates the analysis of large microsatellite datasets.
  • Accurate genotype assignment is essential for the validity of study findings.

Purpose of the Study:

  • To highlight common challenges encountered during microsatellite loci scoring.
  • To provide expert insights for overcoming genotyping errors.
  • To ensure accurate genotype assignment in research studies.

Main Methods:

  • Review of common error sources in microsatellite genotyping workflows.
  • Analysis of potential issues from DNA extraction to database entry.

More Related Videos

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
10:27

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

Related Experiment Videos

Last Updated: May 12, 2026

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells
09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
11:54

Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues

Published on: October 20, 2019

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel
10:27

A Fast Silver Staining Protocol Enabling Simple and Efficient Detection of SSR Markers using a Non-denaturing Polyacrylamide Gel

Published on: April 20, 2018

  • Guidance on understanding data generation chemistry and analysis software.

    • Main Results:

    • Identification of critical points where genotyping errors can occur.
    • Explanation of how various procedural issues can lead to erroneous genotype assignments.
    • Emphasis on the necessity of integrating knowledge of lab chemistry and analysis software.

    Conclusions:

    • Awareness of potential errors is key to reliable microsatellite genotyping.
    • Researchers must critically evaluate automated scoring results.
    • Combining technical knowledge with practical experience ensures data integrity.