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Microelectrode Impalement Method to Record Membrane Potential from a Cannulated Middle Cerebral Artery
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Published on: July 2, 2019

Calcium-sensitive mini- and microelectrodes.

Roger C Thomas1, Donald M Bers

  • 1Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3EG, United Kingdom. rct26@cam.ac.uk

Cold Spring Harbor Protocols
|April 3, 2013
PubMed
Summary
This summary is machine-generated.

Calcium-selective electrodes offer a valuable method for measuring ionized free calcium concentration ([Ca(2+)]) in biological solutions. Their utility complements newer optical probes, providing distinct advantages for researchers.

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Area of Science:

  • Biophysical Chemistry
  • Electrophysiology
  • Analytical Chemistry

Background:

  • Ion-selective electrodes (ISEs) are established tools for measuring ionized free calcium concentration ([Ca(2+)]).
  • Ca(2+)-sensitive macroelectrodes and minielectrodes are commercially available for various solution volumes.
  • Ca(2+)-sensitive microelectrodes (CaSMs) offer intracellular and extracellular measurement capabilities.

Purpose of the Study:

  • To re-emphasize the utility and complementary nature of Ca(2+)-selective electrodes.
  • To highlight the advantages and disadvantages of ISEs compared to optical calcium probes.
  • To provide guidance for researchers with electrophysiological experience.

Main Methods:

  • Utilizing Ca(2+)-sensitive macroelectrodes for large biological solution volumes.
  • Employing custom-made or commercial minielectrodes for smaller sample volumes.
  • Discussing the application of CaSMs for cellular calcium measurements.

Main Results:

  • Ca(2+)-selective electrodes remain a practical and effective method for [Ca(2+)] measurement.
  • ISEs offer complementary data to fluorescent and optical calcium probes.
  • Each measurement technique possesses unique strengths and limitations.

Conclusions:

  • Ca(2+)-selective electrodes are a valuable, albeit sometimes overlooked, tool in calcium research.
  • The choice of method (ISEs vs. optical probes) depends on experimental requirements and sample characteristics.
  • Further investigation into the combined use of ISEs and optical methods can enhance calcium concentration analysis.