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Related Experiment Video

Updated: May 12, 2026

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue

Published on: September 5, 2015

How to make calcium-sensitive microelectrodes.

Roger C Thomas¹, Donald M Bers

¹Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 3EG, United Kingdom. rct26@cam.ac.uk

Cold Spring Harbor Protocols

|April 3, 2013

Summary

This summary is machine-generated.

Calcium-sensitive microelectrodes (CaSMs) offer direct pCa measurement without added buffering or expensive equipment. This protocol details their fabrication and use, addressing potential challenges for researchers.

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Area of Science:

Biomedical Engineering
Electrophysiology
Cell Biology

Background:

Direct measurement of intracellular or extracellular calcium ion concentration (pCa) is crucial for understanding cellular processes.
Existing methods may involve complex procedures or significant buffering effects.
Calcium-sensitive microelectrodes (CaSMs) provide a direct measurement approach.

Purpose of the Study:

To describe a protocol for fabricating and utilizing calcium-sensitive microelectrodes (CaSMs).
To highlight the advantages of CaSMs, such as direct measurement and minimal buffering.
To address practical considerations and potential issues associated with CaSM use.

Main Methods:

Detailed protocol for the construction of CaSMs.
Guidance on the experimental setup, including the use of a reference electrode.
Discussion of electrode tip diameter requirements (>1 μm).

Main Results:

CaSMs enable direct pCa measurement at the electrode tip.
The method avoids adding to cellular buffering capacity.
The technique does not necessitate expensive instrumentation.

Conclusions:

CaSMs are a viable tool for direct pCa measurement in various biological compartments.
The protocol provides a framework for CaSM fabrication and application.
Potential challenges like fabrication time, reference electrode requirement, and leakage are acknowledged and discussed.