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[Development of real-time fluorescent quantitative PCR method for detecting Bombyx mori cytoplasmic polyhedrosis
Ping Wu1, He-Ying Qian, Xi-Jie Guo
1Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang 212018, China. wp4114@163.com
Abstract:
A pair of specific primers were designed according to the published 118 bp conserved sequence of polyhedrin gene of Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) in GenBank and a serial dilutions of a recombinant plasmid were prepared and used to generate standard curves, to establish a real-time fluorescent quantitative PCR method for detection of BmCPV. The results showed that the linear relationship between virus concentration (X) and Ct (Y) was Y = -3. 582 lgX + 38.748 with the correlation coefficient R2 = 0999. The method was very sensitive, specific and reproducible. It can be applied in the rapid detection of BmCPV and the prevalence investigation of this disease.
