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[Effect of gene optimization on the expression and purification of HDV small antigen produced by genetic engineering]
Jun-Ying Ding1, Qing-Ling Meng, Min-Zhuo Guo
1Institute for Viral Disease Control and Prevention, China CDC, Beijing 102206, China.
Objective:
To study the effect of gene optimization on the expression and purification of HDV small antigen produced by genetic engineering.
Methods:
Based on the colon preference of E. coli, the HDV small antigen original gene from GenBank was optimized. Both the original gene and the optimized gene expressed in prokaryotic cells, SDS-PAGE was made to analyze the protein expression yield and to decide which protein expression style was more proportion than the other. Furthermore, two antigens were purified by chromatography in order to compare the purity by SDS-PAGE and Image Lab software.
Results:
SDS-PAGE indicated that the molecular weight of target proteins from two groups were the same as we expected. Gene optimization resulted in the higher yield and it could make the product more soluble. After chromatography, the purity of target protein from optimized gene was up to 96.3%, obviously purer than that from original gene.
Conclusion:
Gene optimization could increase the protein expression yield and solubility of genetic engineering HDV small antigen. In addition, the product from the optimized gene group was easier to be purified for diagnosis usage.
