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Updated: May 12, 2026

Analysis of Group IV Viral SSHHPS Using In Vitro and In Silico Methods
Published on: December 21, 2019
[Isolation and sequence analysis on virus of HFMD with encephalitis in Yantal city]
Wen-qing Han1, Lian-Feng Gong, Juan Liu
1Yantai Center for Disease Control and Prevention, Yantai 264003, China.
Objective:
To learn about the pathogen spectrum and genetic characterization of HFMD with encephalitis in Yantai city.
Methods:
Stool samples and cerebrospinal fluids (CSF) collected from HFMD with encephalitis cases in Yantai. Virus were isolated from stool samples and identified by fluorescence reverse transcription-polymerase chain reaction. The VPl region was amplified and sequenced from positive specimens. Genetic characterization was identified by sequence analysis.
Results:
Getting 3 virus strains from 10 stool specimens and all of them belong to EV71. The nucleotide and amino acid homogeneity with the representative isolates of C4a were 98%-99% and 98.90%-99.45% respectively.
Conclusion:
The pathogen of HFMD with encephalitis in Yantai city were mainly EV71 wich belong to subgenogroup C4 cluster C4a.
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