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Updated: May 12, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
Published on: December 29, 2021
Toehold-mediated nonenzymatic DNA strand displacement as a platform for DNA genotyping
Dmitriy A Khodakov1, Anastasia S Khodakova, Adrian Linacre
1Flinders Centre for Nanoscale Science and Technology, Flinders University, GPO Box 2100, Adelaide, S.A, 5001 Australia. dmitriy.khodakov@flinders.edu.au
Abstract:
Toehold-mediated DNA strand displacement provides unique advantages in the construction and manipulation of multidimensional DNA nanostructures as well as nucleic acid sequence analysis. We demonstrate a step change in the use of toehold-mediated DNA strand displacement reactions, where a double-stranded DNA duplex, containing a single-stranded toehold domain, enzymatically generated and then treated as a molecular target for analysis. The approach was successfully implemented for human DNA genotyping, such as gender identification where the amelogenin gene was used as a model target system, and detecting single nucleotide polymorphisms of human mitochondrial DNA. Kinetics of the strand displacement was monitored by the quenched Förster resonance energy transfer effect.
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