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Establishment and characterization of a new human cell line derived from ovarian clear cell carcinoma
1Department of Obstetrics and Gynaecology, Chinese University of Hong Kong, Shatin, N.T.
Abstract:
A new cell line, designated OCC1, was established from the ascitic fluid of a patient with a clear cell carcinoma of the ovary. The cell line grew well without interruption for over 12 months and over 80 passages. The doubling times of OCC1 were 36 and 38 hr at the 10th and 40th passages, respectively. Chromosomal analysis of the cell line showed hypertriploidy with modal number around 70-77. Several structural chromosomal abnormalities were consistently found. Electron microscopy revealed that OCC1 produced a basement membrane-like structure in vitro. Histological evaluation of xenografts from OCC1 in the 33th passage implanted and grown in nude (athymic) mice revealed a morphology identical to that of the original tumor.