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T cell receptor variable gene expression: analysis in ragweed-sensitive patients during allergen exposure
D A Burton1, G M Shepherd, G W Siskind
1Department of Medicine, Cornell University Medical College, New York, N.Y.
International Archives of Allergy and Applied Immunology
|January 1, 1990
Summary
This study investigated T cell receptor (TCR) V gene usage during an environmental allergen response. Researchers found no consistent shifts in peripheral blood T cells, suggesting changes may occur at tissue sites or involve unmeasured cell populations.
Area of Science:
- Immunology
- Molecular Biology
Background:
- T cell receptor (TCR) V gene usage is crucial for adaptive immunity.
- Previous studies indicated stable TCR V gene usage in peripheral blood T cells (PBT) of healthy individuals.
Purpose of the Study:
- To investigate potential shifts in TCR V gene usage during an immune response to an environmental antigen (ragweed).
- To determine if allergen exposure alters the composition of circulating T cell populations.
Main Methods:
- Utilized four monoclonal antibodies (MAbs) to detect specific TCR V beta chain regions (V beta 5, 6, 8, 12) on PBT via cytofluorography.
- Monitored TCR V gene usage in 12 atopic patients over a 6-month ragweed season.
Main Results:
- No consistent shifts in TCR V gene usage were observed that correlated with the ragweed season.
- Patients were divided into two groups: one with stable T cell populations and another with significant temporal variation, particularly in Ti3a+ T cells (V beta 8).
Conclusions:
- Allergen exposure did not induce consistent, detectable shifts in peripheral blood TCR V gene usage.
- Observed variations suggest that immune responses might involve localized changes at antigen exposure sites or alterations in T cell subpopulations not assessed by the employed reagents.