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Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

Separation of Sister Chromatids

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...

The Spindle Assembly Checkpoint

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

Cohesins

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...

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Related Experiment Video

Updated: May 12, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

Published on: January 14, 2016

ASURA (PHB2) interacts with Scc1 through chromatin.

I Equilibrina¹, S Matsunaga, A Morimoto

¹Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan.

Cytogenetic and Genome Research

|April 4, 2013

Summary

This summary is machine-generated.

Prohibitin 2 (ASURA) protein interacts with the cohesin complex subunit Scc1, aiding in sister chromatid cohesion. ASURA is crucial for maintaining cohesin at early mitosis, suggesting a protective role.

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Last Updated: May 12, 2026

Examination of Proteins Bound to Nascent DNA in Mammalian Cells Using BrdU-ChIP-Slot-Western Technique

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Published on: January 14, 2016

CRISPR-Mediated Reorganization of Chromatin Loop Structure

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Published on: March 9, 2022

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

Sister chromatid cohesion, regulated by the cohesin complex, is vital for accurate chromosome segregation during cell division.
Prohibitin 2 (PHB2), also known as ASURA, is a multifunctional protein previously implicated in sister chromatid cohesion.

Purpose of the Study:

To elucidate the mechanism by which ASURA participates in sister chromatid cohesion.
To investigate the interaction between ASURA and the cohesin complex.

Main Methods:

Co-immunoprecipitation assays to detect in vivo interactions between ASURA and cohesin subunits.
Chromatin association analysis throughout the cell cycle.
Fluorescent ubiquitination-based cell cycle indicator (Fucci) system to assess ASURA's role in mitosis.

Main Results:

ASURA directly interacts with the cohesin subunit Scc1 in vivo.
ASURA exhibits cell cycle-dependent chromatin association, mirroring Scc1 localization.
ASURA is essential for cohesin maintenance during early mitosis, with its PHB domain mediating chromatin targeting.

Conclusions:

ASURA plays a critical role in regulating sister chromatid cohesion by binding to chromatin and interacting with Scc1.
ASURA may protect cohesin complexes, ensuring faithful chromosome segregation.